Can I use a pci-e x16 slot for multple cards?

The slot might be the same, but you have to configure the CPU to treat it as a CXL slot instead of a PCIe slot That obviously requires the CPU

While you should prefer the first slot, in most gaming workloads you're probably fine whichever slot you use That guy does a lot more

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does it matter what pcie slot i use for gpu 2 reasons, your cpu doesn't support it or you have a card in the pcie slot that shares lanes with the gpu D